FLIKA Plugins

Plugins extending FLIKA (Fluorescence Image Analysis) for advanced microscopy workflows.

FLIKA: flika-org.github.io GitHub: github.com/gddickinson/flika_plugins

Available Plugins

thunderSTORM

Single Molecule Localization Microscopy analysis.

Features: - Super-resolution image reconstruction - Localization precision analysis - Drift correction - Batch processing

SPT Batch Analysis

Automated particle tracking for large datasets.

Features: - Multiple tracking algorithms - U-Track integration - Interpolation and gap-closing - Comprehensive export options

Cell Edge Movement

Membrane dynamics and protein localization analysis.

Features: - Edge detection and tracking - Velocity field calculation - Protein colocalization - PIEZO1 analysis tools

Mask Editor

Interactive ROI and mask editing tool.

Features: - Frame-by-frame editing - Boolean and morphological operations - Multiple selection tools - Export to multiple formats

Installation

# Install FLIKA
pip install flika

# Clone plugins
git clone https://github.com/gddickinson/flika_plugins

# Copy to FLIKA plugins directory
cp -r flika_plugins/* ~/.flika/plugins/

Development

All plugins follow FLIKA's plugin architecture with: - __init__.py - Module initialization - plugin_main.py - Core functionality - info.xml - Plugin metadata - about.html - Plugin description

Detailed documentation for each plugin available via links above.